The methanolic extract of the marine sponge Raspilia hirsute collected from Keomun Island resulted in three types of sterols: a mixture of (24S)-Poriferasta-5, 25-diene-$3eta$, 24-diol and (24R)-Stigmasta-5, 25-diene-$3eta$, 24 -diol (1), 25,26,27-Trinorcholest-5-en-$3eta$,24-diol (2), and Pregn-5-en-20-on-$3eta$-ol (3). The isolation and structural determination of these sterols are reported here. Compound 1 showed moderate cytotoxicity against human Leukemia cell line K562.