This paper presents a method for detecting tumors on chicken carcasses by fusion of hyperspectral fluorescence and reflectance images. Classification of normal skin and tumor is performed by the image obtain 어 from optimal band ratio which minimizes the overlapping area of PDFs for normal skin and tumor. This method yields four feature images, each of them represents the ratio of two intensity values from a pixel. Classification is achieved by applying ISODATA to each pixel from the feature images. For the analysis of reflectance image, band selection method is proposed based on the information quantity, many effective features are acquired for the classification by defining the linear transformation selecting the projection axis, accordingly, accurate interpretation of images is possible in the reflectance image and automatic feature selection method is realized. Feature images from reflectance images are also classified by ISODATA and combined with the result from fluorescence images. Experimental result indicates that improved performance in term of reducing false detection rate is observed.