Dental personnels faced risks of infection in the clinic. For infection control, recognition and practice of dental personnels are important factor. This study was performed to investigate the recognition and practice of dental hygiene for infection control and infection waste control. A stratified convenience sample of dental hygienists in dental health-care settings. The major finding of the present study are as follows: 1. The existence of education about Standard Precaution and low of infection waste storage was higher dental hospital than dental clinic. 2. The degree of practice in the storage of dental wastes was low in absorbent cotton and body tissue exclude damage waste. And the degree of practice in the disposal of dental wastes was high in all three. 3. Practice in the storage of dental waste was higher dental hospital than dental clinic. 4. At the conclusion of this investigation, systematic refresher training of infection control should be prepared by campaign an various media, Dental health care workers should be encouraged to practice those action items from training. For successful implementation of infection control in every dental health-care settings, it is highly demanded as well that development of effective safe-guard tools, stategic support, and standardized action items against infection problems.