Purpose: This study conducts quantitative evaluation or structural and functional characteristics or normal skeletal muscle with ultrasound image and surface electromyography, and is to provide basic materials for utilizing ultrasound image analysis in physical therapy diagnosis and assessment of skeletal muscle. Methods: Measurement of three stages was conducted with 88 normal adults between their twenties and seventies, correlations and differences using collected data according to age and gender were compared and correlations among measured items were analyzed and then the following conclusions were obtained. Results: Analysis of ultrasound image of normal skeletal muscle showed that density, median frequency had the closest relations with age. In addition, it was found that there were high correlations between density explaining structural characteristics of skeletal muscle and median frequency explaining functional characteristics. Conclusion: Analysis of ultrasound image makes complex evaluation of structure and function of skeletal muscle possible when it is connected with functional evaluation method using physical measurement surface electromyography as well as quantitative evaluation of structural changes of skeletal muscle and is effective in complementing physical therapy diagnosis centering around functionality evaluation.