This study investigated the relationship between land cover and the water quality variables in the rivers, which are located in the Yamaguchi prefecture of West Japan. The study area included 12 catchments covering $5,809;Km^2$. pH, dissolved oxygen, suspended solid, E. coli, total nitrogen and total phosphorus were considered as river water quality variables. Satellite data was applied to generate land cover map. For linking alterations in land cover (at whole catchment and buffer zone levels) and the river water quality variables, multiple regression modeling was applied. The results indicated that non-spatial attribute (%) of land cover types (at whole catchment level) consistently explained high amounts of variation in biological oxygen demand (72%), suspended solid (72%) and total nitrogen (87%). At buffer zone-scale, multiple regression models that were developed to represent the linkage between the alterations of land cover and the river water quality variables could also explain high level of total variations in suspended solid (86%) and total nitrogen (91%).