30일 동안 15 psu에서 순화 사육한 양식 넙치(평균전장: 20.8cm,평균체중: 96.1g)를 사용하여 염분변화에 따른 혈액 학적 변화 (cortisol, glucose, $Na^+$, $Cl^-$, $K^+$, AST, ALT, hematocrit, RBC, hemoglobin) 및 생존율을 조사하였다. 사육수를 실험개시시 15 psu로부터 0 psu(group I, G I)와 35 psu(group II, G II)로 30분 이내에 교환하였다. 5일 동안 유지하였으며, 다시 사육수를 0 psu로부터 35 psu(G I)와 35 psu에서 0 psu(G II)로 교환하였다. G I구는 cortisol, glucose, $Na^+$, $Cl^-$, AST및 생존율 등에서 일반적인 stress 경향을 보였다. G II구는 35 psu에서 0 psu로 교환한 240시간째에 cortisol, glucose 및 AST에서 유의적으로 높아진 값을 보였다. 두 실험구에서 생존율은 35 psu에서보다 0 psu에서 낮아지는 것으로 나타났다. 본 연구 결과 고염분과 저 염분변화에 따른 cortisol, glucose, $Na^+$, $K^+$, $Cl^-$및 생존율에 대한 기초자료로 유용할 것이다.
We examined the effect of salinity on hematological changes and survival in the cultured olive flounder, Paralichthys olivaceus. Fish (mean length: 20.8 cm, mean weight: 96.1 g) were abruptly (within 30 min) exposed to salinities from 15 to 0 psu (Group I, G I) and to 35 psu (Group II, G II). After maintain during days 5, the fish were again changed from 0 to 35 psu (G I) and from 35 to 0 psu. We measured cortisol, glucose, $Na^+$, $Cl^-$, $K^+$, aspartate amino-transferase (AST), and alanine amino-transferase (ALT), hematocrit, RBC, hemoglobin and survival during the 10-days test period. Our results in G I showed that olive flounder exhibit "typical" physiological responses (in cortisol, glucose, $Na^+$, $Cl^-$, AST and survival) to the stress induced by salinity changes. In G II, olive flounder were significantly different in cortisol, glucose, and AST at 240 hours. The survival in two groups were reduced in 0 psu than 35 psu during the experimental period. This research provides baseline data on cortisol, glucose, $Na^+$, $K^+$, $Cl^-$ and survival for hypo or hyper-salinity changes.
