This paper deals with the applicability of long-gauge deformation fiber optic sensors (FOS) to prestressed concrete structures. A main motivation is the desire to monitor the deflection of the railway bridges without intervenes of the signal intensity fluctuations. A 25 m long, 1.8 m deep PSC girder was fabricated compositely with 22 cm thick reinforced concrete deck. Two pairs of 3 m long-gauge sensors are attached to the prestressed concrete girder with parallel topology. Using the relationship between curvature and vortical deflection and the quadratic regression of curvatures at the discrete point, it is possible to extrapolate the deflection curve of the girder. The estimated deflection based on the developed method is compared with the results using conventional strain gauges and LVDTS. It has been demonstrated that the proposed instrumentation technique is capable of estimating the vertical deflection and neutral axis position of the prestressed concrete girder up to weak nonlinear region.