Recently, direct load analysis using ship motion program is required to confirm structural safety for the Post-Panamax class large container carrier. However, there is no exact comparative study data for structural response between 20 and 30 wave load. So, in this paper, to compare the hull girder stress response between 20 versus 3D wave load calculation method, direct load analysis and global F.E analysis have been performed for three kinds of large container vessels using each 20 and 30 wave load calculation program. The results of 2D wave load RAO(Response Amplitude Operator) of each dominant load parameter(vertical, torsional and horizontal moment) are generally bigger than that of 30 results, especially in vertical wave bending moment. And the results of structural analysis based on the equivalent design wave method shows that there is a big difference in view of stress, but the stress distribution is very similar for each wave load case.