Purpose: The purpose of this study were to analyze fatigue patterns of the rectus femoris muscle by isometric contraction. Methods: Twenty healthy subjects(10 male, 10 female) participated in this study. Maximal voluntary isometric contraction(MVIC) was measured by the dynamometer. Muscle activity was recorded from the rectus femoris muscle. During the experiment, the subject was seated in the chair. The measured items, median frequency(MDF) and root mean square (RMS), were collected from the surface EMG. All data were analyzed using repeated measures ANOVA. Results: There was significant difference of MVIC between male and female. Endurance time was significant difference by the level of MVIC but no significant in the main effect(sex) and interaction effect. The MDF and fatigue index were significant differences in the interaction effect. The RMS was not significant difference in both of main and interaction effect. The muscle fatigue patterns of female was greater than male after 60% MVIC. Conclusion: This study showed that sex differences of muscle fatigue were started from 60% MVIC.