We investigated the factors that affect biomass production from Anoectochilus formosanus in a bioreactor system. Those factors included inoculum size, initial sucrose concentration, media supplements, photosynthetic photon flux density (PPFD), and culturing methods. An inoculum size of $8;g;L^{-1}$ was most suitable for shoot proliferation; biomass accumulation was optimized when the medium was supplemented with 3% sucrose compared with sucrose-free media or those containing concentrations of 6% or 9%. This accumulation also was enhanced under a PPFD of $50;{mu}mol;m^{-2};s^{-1}$. Likewise, the addition of coconut water ($50;mL;L^{-1}$) plus activated charcoal ($0.5;mg;L^{-1}$) to our Hyponex medium proved most beneficial. Comparative studies among three bioreactor systems - continuous immersion, raft (net), and temporary immersion (the ebb and flood system) - revealed that shoot proliferation and biomass accumulation were more efficient when culturing was performed under continuous immersion.