Identification and quantification of allantoin in rice grain of selected varieties were investigated. Allantoin was isolated from Jeokjinjubyeo, and its structure has been elucidated on the basis of spectral data. Allantoin was extracted with a 70% acetone and analyzed by high performance liquid chromatographic methods without a previous chemical derivatization. The concentration of allantoin in selected rice varieties was in a narrow range from 2 to 18 mg per 100 g of brown rice. The highest content was detected in Heugkwang with 18.6 mg per 100 g brown rice, while the lowest was 2.59 mg in Yeomyung.