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Effect of Culture Conditions and Freezing Methods on Developmental Competence of Hanwoo Embryos Cultured In Vitro
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  • Effect of Culture Conditions and Freezing Methods on Developmental Competence of Hanwoo Embryos Cultured In Vitro
  • Effect of Culture Conditions and Freezing Methods on Developmental Competence of Hanwoo Embryos Cultured In Vitro
저자명
Song. S.H.,Min. C.S.,Son. G.D.,Rho. C.W.,Kang. Y.S.,Park. C.S.,Kong. I.K.
간행물명
韓國受精卵移植學會誌
권/호정보
2007년|22권 4호|pp.251-255 (5 pages)
발행정보
한국수정란이식학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

This study was carried out to examine on developmental competence of Hanwoo embryos cultured in vitro according to culture conditions and freezing methods. The in vitro developmental competence to blastocyst stage at Day 8 of culture in SOF was significantly (p<0.05) higher than that in CR1aa (30.3% vs. 18.4%). The in vitro developmental rate of morula and blastocysts cultured in group culture was significantly (p<0.05) higher than that in individual culture (41.4% and 36.0% vs. 21.1% and 10.5%, respectively). The cell number of Day 8 blastocysts in group culture was significantly (p<0.05) higher than that in the individual culture ($120.1{pm}12.8;vs.;94.1{pm}12.1$, respectively). The survival rates of frozen-thawed balstocysts that were exposed in 1.5 M ethylene glycol or 1.5 M ethylene glycol containing 0.1 M sucrose were 77.5% and 78.7%, respectively. The survival rates of blastocysts cultured for 48 h in slow freezing and vitrification was not significantly different (73.3 and 74.0%). In conclusion, in vitro developmental competence of bovine embryos was influenced on the culture medium (SOF) and culture method (Group culture). Survival rate of frozen-thawed of bovine embryos was not influenced on freezing solutions and freezing methods.