In this study, we propose an RFID based container terminal operation process. Field tests are conducted for 900 MHz (truck identification) tag and 433 MHz RFID (container identification/e-seal) tag at an Incheon container terminal to verify possibility of applying RFID in the container terminal gate. To evaluate effect of applying RFID in the container terminal operation, simulation models for current and RFID based container terminals are developed using ARENA. Simulations are carried out using industrial field data and the result indicates that performance of the container terminal can be prominently improved after RFID application.