It is important to measure the rotational accuracy of lathe spindle as it affects to the qualities of all machines machined by the lathe using in industries. The bad rotational accuracy of lathe spindle are caused mainly by wearness of the spindle in using and quality of spindle when machining and using low level bearings. It occurs especially in case of lathes because the cutting force acting to work-piece act on one side to the spindle not to both sides symmetrically. Therefore in this study, constructing experimental apparatus for measuring of rotational accuracy by using eddy current type gap sensors AEC5706PS and sensors, s-06LN, data acquisition board DT9834(USB type) and software for data acquisition, DT Measure Foundry ver. 4.0.7 etc., error data acquired in the rotational accuracy test of lathe spindle are analysed in plots and statistical treatments.