The puncture properties of short-fiber reinforced rubber were investigated as functions of fiber aspect ratio(AR: length of fiber/diameter of fiber), fiber content, specimen size and testing velocity. The puncture stresses of the matrix and short-fiber reinforced rubber decreased with specimen size, and increased with testing velocity at same specimen size. As the fiber AR increased the puncture stress at given fiber content also increased. The problem of the specimen shape was investigated by the comparison of the tensile strength with puncture stress. The forces acting in the membrane wall of the matrix and the short-fiber reinforced rubber showed a similar data regardless of specimen size. And those increased with testing velocity at same specimen size. As the fiber AR increased the force acting in the wall at given fiber content also increased. Overall, it was found that the specimen size, testing velocity had an important effects on the puncture properties.