Phytochemical investigation of the hexane and $CH_2Cl_2$ extracts of Erythrina stricta roots and E. subumbrans stems led to the isolation of six pterocarpans, one flavanone, one isoflavone, two alkaloids, five triterpenes, six steroids and alkyl trans-ferulates. The structures of all known compounds were determined on the basis of spectroscopic evidence. Sophoradiol (15), a mixture of stigmast-4-en-3-one (19) and stigmasta-4,22-dien-3-one (20), lupeol (21), cycloeucalenol (22), a mixture of $3{eta}$-hydroxystigmast-5-en-7-one (23) and $3{eta}$-hydroxystigmast-5,22-dien-7-one (24) and melilotigenin C (25) were first isolated from the genus Erythrina. The isolated compounds were evaluated for antiplasmodial activity, antimycobacterial activity and cytotoxicity. Among the tested compounds, 5-hydroxysophoranone (8) exhibited the highest antiplasmodial activity against Plasmodium falciparum $(IC_{50};2.5{mu}g/mL)$. Compound 8, erystagallin A (5), erycristagallin (7) and erysubin F (10) showed the same level of antimycobacterial activity against Mycobacterium tuberculosis $(MIC;12.5{mu}g/mL)$. For cytotoxicity, erybraedin A (2) showed the highest activity against the NCI-H187 and BC cells ($IC_{50}$ 2.1 and $2.9{mu}g/mL$, respectively), whereas 10 exhibited the highest activity against the KB cells $(IC_{50};4.5{mu}g/mL)$.