A heparin/polypyrrole (PPy) composite, an electrical conducting polymer, was designed to enhance the interactions between a gold-coated matrix and nerve cells, with the cell (PC12 cells) interactions investigated under different conditions, both with and without electrical stimulation. The heparin concentration in the composites increased with increasing current density under the preparation condition, indicating that the heparin concentration in the composite could be controlled by managing the current density. Optical imaging showed that PC12 cells well attached to the PPy surfaces covered with heparin, but were poorly interacted to PPy surfaces without the heparin and gold coated matrix. The neurite length of the PC12 cells on the surfaces with an electrical stimulation (100 mV for 1 h) significantly increased, with a median length of $77.5{mu}m;$ whereas, that without electrical stimulation was $10{sim}20{mu}m$. Therefore, the heparin/polypyrrole (PPy) composite may provide insight for the development of an ideal nerve guidance channel.