In situations of spatio-temporal perception about visuo-auditory stimulus, researches propose optimal integration hypothesis that perceptual process is optimized to the interaction of the senses for the precision of perception. So, when the visual information considered generally dominant over any other sense is ambiguous, the information of the other sense like auditory stimulus influences the perceptual process in interaction with visual information. Thus, we performed two different experiments to certain the conditions of the interacting senses and influence of the condition. We consider the interaction of the visuo-auditory stimulation in the free space, the color of visual stimulus and sex difference of testee with normal people. In first experiment, 12 participants were asked to judge the change in the frequency of audio-visual stimulation using a visual flicker and auditory flutter stimulation in the free space. When auditory temporal cues were presented, the change in the frequency of the visual stimulation was associated with a perceived change in the frequency of the auditory stimulation as the results of the previous studies using headphone. In second experiment, 30 male and 30 female were asked to judge the change in the frequency of audio-visual stimulation using a color of visual flicker and auditory flutter stimulation. In the color condition using red and green. Both male and female testees showed same perceptual tendency. male and female testees showed same perceptual tendency however, in case of female, the standard deviation is larger than that of male. This results implies that audio-visual asymmetry effects are influenced by the cues of visual and auditory information, such as the orientation between auditory and visual stimulus, the color of visual stimulus.