This paper introduces the algorithm developed to measure the local temperature of phases in the non-isothermal two-phase flow by using micro-thermocouple, which was verified using an optical chopper and a laser. The micro-thermocouple, whose outer diameter of which is 12.7 $mu$m, consists of a couple of alumel-chromel wire (K-type). The hot-junction, a part of the sensor used to for measuring temperature, is fabricated by hot-wire shapes. The response time of the micro-thermocouple, which was measured by dynamic calibration method, was several milliseconds in flow condition. An algorithm to calculate the temperature of each, which is based on the response time of micro-thermocouple and the exponential regression method, was developed. And the developed algorithm was verified through experiments using an optical chopper and a laser.