The mechanism of power transmission from motor torque to stem thrust and the operation characteristic of each stroke position are analyzed using the diagnostic signal, and effects of differential pressure on the performance of motor operated flexible wedge gate valve are investigated. Test facility consists of 76 mm motor operated valve(flexible wedge type), pump and pipe system. Static and dynamic test are performed separately, and two differential pressure conditions are applied in the dynamic test. To evaluate the performance of valve, test signals for the torque, thrust, current, voltage and stroke length are acquired by using UDS which is diagnosis device for motor operated valve, and each diagnostic signal is analyzed and compared. The characteristic of valve performance factors such as stem factor, rate of loading, valve factor, are evaluated, and these factors are found to be severely influenced by the fluid differential pressure.