High speed machining has become the main issue of metal cutting. Due to increase of the rotational speed of the spindle, problems such as the run-out errors and reduced stiffness must be overcome to improve the machining accuracy. In order to solve the problems, it is important to determine the appropriate clamping unit and tooling system. This paper presents an investigation into an analysis of static stiffness in the main spindle interface. Finite element analysis is performed by using a commercial code ANSYS according to variation of cutting force, clamping force and rotational speed. From the finite element results, it is shown that the rotational speed and clamping force mostly influence on the variation of the static stiffness in the main spindle interface.