This paper deals with speed-sensorless control of DC servo motor using Neuro-Fuzzy Observer. DC servo motor has very low rotor inertia and excellent response characteristic and it is very useful to control torque and speed. It is easy to detect the voltage and current and resolver or encoder is used to measure a rotor speed. But it has a limit as a driving speed to detect speed precisely. So it is problem to improve the performance of the driving system. To solve this problem, it is studied to detect a speed of DC servo motor without sensor. In particular, study on the method to estimate the speed using the observer is performed a lot. In this paper, the gain of the observer is properly set up using the Neuro-Fuzzy control and Neuro-Fuzzy Observer that have a superior transient characteristic and is easy to implement compared the existing method is designed. It calculates the differentiation of the rotor current directly using the rotor current measured in the DC servo motor and estimates the speed of the rotor using the differentiation. Proposed speed sensorless control method is performed using the estimated speed. Also, it is proved feasibility of the proposed observer from the comparison tested a case with a speed sensor and a case without a speed sensor which used a highly efficient drive and 200[w] DC servo motor starting system.