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Differential Stringent Responses of Streptomyces coelicolor M600 to Starvation of Specific Nutrients
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  • Differential Stringent Responses of Streptomyces coelicolor M600 to Starvation of Specific Nutrients
  • Differential Stringent Responses of Streptomyces coelicolor M600 to Starvation of Specific Nutrients
저자명
Ryu. Yong-Gu,Kim. Eun-Sook,Kim. Dae-Wi,Kim. Sung-Keun,Lee. Kye-Joon
간행물명
Journal of microbiology and biotechnology
권/호정보
2007년|17권 2호|pp.305-312 (8 pages)
발행정보
한국미생물생명공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

This study focused on the involvement of the unusual nucleotide (p)ppGpp, a stringent factor, during the morphological and physiological differentiation of Streptomyces coelicolor. Two genes, relA and rshA, were disrupted to demonstrate the roles of the stringent factor in the differentiation. The intracellular concentration of (p)ppGpp in the wild-type (M600) and disrupted mutants was measured in relation to the intentional starvation of a specific nutrient, such as carbon, nitrogen, and phosphate or the in situ depletion of nutrients in a batch culture. As a result, it was found that the morphological characteristic of the ${Delta}relA$ mutant was a bld phenotype forming condensed mycelia, whereas the ${Delta}rshA$ mutant grew fast-forming spores and straightforward mycelia. In both mutants, the production of actinorhodin (Act) was completely abolished, yet the undecylprodigiosin (Red) production was increased. Intracellular (p)ppGpp was detected in the ${Delta}relA$ mutant in the case of limited phosphate, yet not with limited carbon or nitrogen sources. In contrast, (p)ppGpp was produced in the ${Delta}rshA$ mutant under limited carbon and nitrogen conditions. Therefore, (p)ppGpp in S. coelicolor was found to be selectively regulated by either the RelA or RshA protein, which was differentially expressed in response to the specific nutrient limitation. These results were also supported by the in situ ppGpp production during a batch culture. Furthermore, it is suggested that RelA and RshA are bifunctional proteins that possess the ability to both synthesize and hydrolyze (p)ppGpp.