The electro-acoustic guitar pickup converts the vibration of strings to the electric signals, and delivers them to an amplifier. The vibration of the strings is transferred to the piezoelectric material through the saddle. This paper aims to improve sound quality for electro-acoustic guitars through the finite element analysis of the saddle. Firstly, the conventional pickup is modeled and analyzed with a commercial program called ANSYS. It is obvious that there exists interference phenomenon of stress. A structural modification of the pickup is performed, based on the beam theory. The modified structure is modeled and analyzed. Finally, the fabricated structures are subjected to the measurements and compared with the conventional pickup. It can be concluded that the interference with the modified structure is much less than that with the conventional structure, and that the sound quality is improved with the modified structures.