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Development of a Virus Concentration Method and its Application for the Detection of Noroviruses in Drinking Water in China
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  • Development of a Virus Concentration Method and its Application for the Detection of Noroviruses in Drinking Water in China
  • Development of a Virus Concentration Method and its Application for the Detection of Noroviruses in Drinking Water in China
저자명
Liu. Junyi,Wu. Qingping,Kou. Xiaoxia
간행물명
The journal of microbiology
권/호정보
2007년|45권 1호|pp.48-52 (5 pages)
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한국미생물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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A new procedure for the concentration of nonoviruses from water samples has been developed. This procedure (calcium flocculation-citrate dissolution method) uses the following steps: virus flocculation formed by treatment with 1 M $CaCl_2$ and 1 M $Na_2HPO_4$, virus release by sodium citrate dissolution (0.3 M Na citrate, pH 3.5), and virus re-concentration by ultrafiltration. When reverse transcription (RT)-PCR was performed after the procedure, the overall detection sensitivity for seeded noroviruses in a one liter drinking water sample was as low as 1 RT-PCR unit, which is equal to a $10^{-6}$ dilution of the virus sample. This approach showed at least a 5-fold-higher sensitivity than the current method with its three steps of adsorption-elution-concentration. The newly developed procedure was used to test different brands of bottled drinking water from China for putative contamination with noroviruses. A total of 144 samples were analyzed; all of the samples were negative for norovirus specific nucleic acids.