This paper considers about the tilt measurement of lens module with multiple lenses. The tilt between lenses in lens module and barrel or between image sensor and barrel can be measured precisely with the proposed algorithm. The magnitude and direction of the tilt vector of lens and image sensor can be measured from the best focal surface. The selecting and setting of image sensor, test chart, image sensor centering to lens module, axis align, focus measure method are also explained to get highly precise tilt results. The proposed algorithm is verified with the lens module inspection system we developed, and the experimental results show that the tilt measure proposed in this paper is robust and precise. With the proposed tilt measurement algorithm, the tilt of an image sensor and any other lens which intermediates light can be measured.