This paper suggested the best work conditions including short break time and the number of mouse clicks on the computer work for the prevention of MSDs on VDT work. Fatigue measures included EMG based parameters. The short break time conditions are grouped into 7, 15, and 30 seconds after every work for 10 min and the number of mouse clicks are divided into 10, 20, and 30 clicks/min. The result of the ANOVA of the shift value of %MVC(Maximum Voluntary Contraction) showed the following: 1) There was a considerable difference as regards to the break time except the number of mouse clicks on the upper trapezius muscle(p$<$0.05). The best conditions were shown in 15 sec after every 10 min and 30 clicks/min. 2) There were considerable differences as regards to the number of mouse clicks except the break time on the extensor digitorum muscle and extensor carpi ulnaris muscle(p$<$0.05). The best conditions were shown in 7 sec after every 10min and 10 clicks/min.