This paper is to investigate the characteristics of exhaust emissions and nano-particle emission from diesel passenger vehicle according to using biodiesel fuel as an alternative fuel. In this work, the particulate matters (PM) of exhaust emissions in diesel engine were investigated by number of particles and mass measurement. The mass of the total PM was measured using the standard gravimetric measurement method, the total number concentrations were measured on a ECE15+EUDC driving cycle using Condensation Particle Counter (CPC). Total PM emission was reduced $2{sim}38%$ and number concentration was reduced $1{sim}27%$ according to increasing blended ratio of biodiesel with diesel fuel. Total PM emission was reduced more than particle number emission because volatile particles were measured in total PM but were not measured in particle number emissions.