Cyclodextrin is a cyclic oligosaccharid material which shows an ability to incorporate organic guest molecules inside their cavity area. Thus, this ${eta}-cyclodextrin$ treatment on fiber substrates may provide the changed surface characteristics of the substrates such as solubility, chemical reactivity and spectral property. In this context, the aim of this present work is to make a bridge connection using hetero-bi-functional reactive dye between fiber substrates and ${eta}-cyclodextrin$. In addition, the corresponding Berberine inclusion behaviors into the inner cavity of ${eta}-cyclodextrin$ was examined. The %exhaustion of Berberine inclusion as a guest molecule within the ${eta}-cyclodextrin$ was measured using UV-Vis spectrophotometer. The findings showed that the %exhaustion of Berberine inclusion increased with increasing the prepared dye bridge compound and ${eta}-cyclodextrin$ host material.