The probability and the reliability-based seismic performance evaluation procedure proposed in the FEMA-355F was applied to a reinforced concrete moment frame building in this study. For the FEMA procedure, which was originally developed for steel moment frame structures, to be applied to other structural systems, the capacity should be re-defined and the factors reflecting the uncertainties related to capacity and demand need to be determined. To perform the evaluation procedure a prototype building was designed per IBC 2003, and inelastic dynamic analyses were conducted applying site-specific ground motions to determine the parameters for performance evaluation. According to the analysis results, distribution of the determined capacities turned out to be relatively smaller than that of the demands, which showed that the defined capacity was reasonable. It was also shown that the prototype building satisfied the target performance since the determined confidence levels exceeded the objectives for both local and global collapses.