This paper presents a method of a line balanced assembly sequence generation based on the verification of a disassemblability and a work time. To derive the disassemblability for a part to be disassembled, first we inference collision free assembly directions by extracting separable directions fur the part. And we determine the disassemblability defined by the separability and stability cost. The separability cost represents a facility of the part disassembly operation, and the stability cost which represents a degree of the stability for the base assembly motion. Based upon the results, we propose a new approach of evaluating work time using neural networks. The proposed assembly sequence generation provides an effective means of solving the line balancing problem and gives a design guidance of planning assembly lay-out in flexible manufacturing application. Example study is given to illustrate the concepts and procedure of the proposed schemes.