The purpose of this research was to investigate the effects of used color and tone on harmony perception by applying contrast coloration of shirt and necktie. This experiment designed by shirt color(red, blue, purple, green), tie color(green or blue tie with red shirt, yellow or red tie with blue shirt, yellow or green tie with purple shirt, purple or red tie with green shirt), shirt tone(vivid, light, dull, dark), tie tone(vivid, light, dull, dark), and perceiver gender(a male, a female). The experimental materials developed for this study were a set of stimulus and response scales, we harmonized those colors of shirts and neckties contrast, then made shirt and necktie tone differently. The subjects of this research were 640 male and 640 female college students. The data was analyzed by using SPS program. Shirt color, tie color, shirt tone, tie tone, perceiver gender influenced on harmony perception independently. Shirt color and tie color, shirt color and shirt tone, shirt color and perceiver gender, tie color and tie tone, tie color and perceiver gender, shirt tone and tie tone influenced on the harmony perception by interaction.