Clerodendron trichotomum Tunberg Leaves (CTL) have been used for centuries in Chinese folk medicine for their anti-inflammatory properties. To investigate the molecular mechanism of anti-inflammation by CTL, we analyzed the regulation of TNF-${alpha}$ expression in RAW 264.7 Cell a key step in inflammation. The effect of CTL on the production and expression of tumornecrosis factor-${alpha}$ (TNF-${alpha}$) was determined by enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). CTL inhibited the production and expression of TNF-${alpha}$ in LPS-stimulated RAW 264.7 cells in a dose-dependent manner. In addition activation of NF-${kappa}$B, which controls TNF-${alpha}$ expression, was inhibited in LPS-stimulated RAW 264.7 cells by CTL in a dose-dependent manner, as demonstrated by an electrophoretic mobility shift assay (EMSA). Furthermore, CTL inhibited activation of NF-${kappa}$B through inhibition of I${kappa}$B degradation, as demonstrated by an western blot analysis of I${kappa}$B-${alpha}$. These results suggest that CTL inhibits the expression of the pro-inflammation gene through the inhibition of NF-${kappa}$B dependent pathway in RAW 264.7 cells.