This study was performed to investigate the discrimination of Angelica gigas, Angelica acutiloba and Angelica sinensis on the treatment of chromaticity and colorfastness. Angelica gigantis root has been used as a Korean traditional medicine for the treatment of woman disease. Natural dyes give us many great benefits, including diversified color, but no pollution. These studies were carried out acetate iron, dichloride copper and alum with a mordant to ramie fabric. The ramie fabric was dyed with Angelica gigas, Angelica acutiloba and Angelica sinensis. The results of experiment showed as follows: In discrimination by dyeing, the colors of Angelica acutiloba and Angelica sinensis were very similar, but that of Angelica gigas was different. There were no differences among colors of materials using non-mordant. But dyeing with iron acetate and copper dichloride were showed dark in Angelica gigas than other angelica species.