The fiber dispersion in fiber-reinferced cementitious composites is a crucial factor with respect to achieving desired mechanical performance. However, evaluation of the fiber dispersion in the composite PVA-ECC (polyvinyl alcohol-engineered cementitious composite) is extremely challenging because of the low contrast of PVA fibers with the cement-based matrix. In the present work, a new evaluation method is developed and demonstrated. Using a fluorescence technique on the PVA-ECC, PVA fibers are observed as green dots in the cross-section of the composite. After capturing the fluorescence image with a charged couple device (CCD) camera through a microscope, the fiber dispersion is evaluated using an image processing technique and statistical tools. In this image processing technique, the fibers are more accurately detected by employing an enhanced algorithm developed based on a discriminant method and watershed segmentation. The influence of fiber orientation on the fiber dispersion evaluation was also investigated via shape analyses of fiber images.