In the present study, naturally generated bubbles were investigated to be sure if they could be adopted as the tracer for PIV techniques. The bubble can be grown from the nuclei melted in the water of tunnel and the size of bubbles is changed through the variation of tunnel pressure. Since the trace ability and appropriate size of tracer are so important for PIV techniques, the characteristics of bubbles as tracer are revealed in terms of trace ability and bubble size with the variation of flow speed and tunnel pressure in this study. In addition, PIV measurements of (low behind a rotating propeller are conducted to confirm the trace ability of bubbles even in a highly vortical flow.