The paper deals with the optimization of runner system in injection molding design. The design objective is to locate gate positions by minimizing both maximum injection pressure at the injection port and maximum pressure difference among all the gates on a product with constraints on shear stress and/or weld-line. The analysis of filling process is conducted by a finite element based program for polymer flow. Micro genetic algorithm (mGA) is used as a global optimization tool due to the nature of inherent nonlinearlity in flow analysis. Four different design applications in injection molds are explored to examine the proposed design strategies. The paper shows the effectiveness of mGA in the context of optimization of runner system in injection molding design.