The purpose of this study was to ascertain the ecological state and problems of eco-bridges on Korean roads. The study was conducted from March to April 2007 by checking ecological factors and wildlife around the eco-bridges and habitats. 8 representative eco-bridges were selected by their bridge shape and condition of location. After field survey, we analyzed ideal trails for wildlife using field data, digital topology maps, satellite images, and land use map. In addition to trail analysis, we identified the propriety of the eco-bridges through Population Viability Analysis. Because of unsuitable location and management, we found that most of the bridges are not proper for wildlife dispersal. Moreover, in some habitats, we could expect that some local populations might be extinct without appropriate treatments.