Objectives : The purpose of this study is to investigate the delayed luminescence-biophoton emission from root of Angelica sinensis D., Angelica acutiloba K., and Angelica pubescens M. These three species of Genus Angelica are now imported from other nations into Republic of Korea. Methods : Randomly selected samples from roots of Angelica sinensis D., Angelica acutiloba K., and Angelica pubescens M. were radiated with 150 W metal halide lamp for 1 minute. After radiation. biophoton emissions of each sample were detected by electron multiplication(EM)-charge coupled device camera. The detected biophoton image was calculated with unit of counts per pixel. Results: The average biophoton emissions of delayed luminescence with EM ratio of $ imes$150 and $ imes$250 were distinguished significantly. The maximum biophoton emissions of delayed luminescence with EM ratio of $ imes$250 were distinguished significantly. Conclusions : These results suggest that biophoton imaging of roots of Angelica sinensis D., Angelica acutiloba K., and Angelica pubescens M. could become the meaningful method for the study of differentiation for these three species of Genus Angelica.