The purpose of the study is to investigate the effect of e-Learning course for kindergarten teachers and to generate suggestions for the revision of the course. To pursue for the purpose of the study, two hundred and ninety two subjects had participated in the quantitative study. The results indicate that the e-learning course was effective in terms of the student achievement, especially for the less privileged students. For the learners who were not majored in education for young children, and who had lower level academic degree enjoyed more benefit from the e-Learning course than their colleagues with higher level academic degrees. Also some issues that should bring some concerns such as the flaws of measurement instrument were discussed. Finally, implications regarding instructional design of e-Learning courses were suggested.