Objectives : This study was conducted to assess the effect of Uhwangchungsimwon on the autonomic nervous system. Methods : Eligible subjects were enrolled from healthy people in their 20s-30s. They were divided into two groups, the UC group (n=22) and the control group (n=20). We monitored ECG from 14:00 to 18:00 in both groups. In the UC group, subjects were administered UC at 15:00. HRV analysis was conducted at 14:00, 16:00, and 17:00 for 512 seconds. Results : The degree of LF/HF ratio change was significantly higher in the UC group at 1 hour after administration. Conclusion : We suggest Uhwangchungsimwon may be useful for stabilization of the autonomic nervous system by activating parasympathetic nerve in healthy people.