The barley ERF-type transcription factor, HvRAF, has an acidic C-terminus domain that contains a hemopexin domain signature-like sequence. As an initial study to investigate the functioning of this domain, we cloned the partial HvRAF gene (pHvRAF) corresponding to the domain into a yeast/E. coli shuttle vector, pYES2. We then introduced the pYES2::pHvRAF recombinant plasmid into yeast (Saccharomyces cerevisiae strain INVSc1). Here, we report that yeast transformants harboring pYES2::pHvRAF show significant tolerance to cadmium stress.