The eco-hydrologic effects of maintenance water supply on Oncheon stream are studied using hydrologic, hydraulic and ecologic models. SWMM (Storm Water Management Model) is used for long-term simulation of runoff quantity and water quality from Oncheon stream watershed. Using the output hydrologic variables from SWMM, HEC-RAS (River Analysis System) is then used to simulate the hydraulics of water flow through Oncheon stream channels. Such hydrologic, hydraulic and water quality output variables from SWMM and HEC-RAS are served as input data to execute PHABSIM (Physical Habitat Simulation) for the purpose of predicting the micro-habitat conditions in rivers as a function of stream flow and the relative suitability of those conditions to aquatic life. It is observed from the PHABSIM results that the weighted usable area for target fishes has the maximum value at $2m^3/s$ of instream flow. However, mid and down stream areas that have concrete river bed and covered region are unsuitable for fish habitat regardless of instream flow increment. The simulation results indicate that the simple maintenance water supply is limited in its effect to improve the ecological environment in Oncheon stream. Therefore, it is imperative to improve water quality and to recover habitat conditions simultaneously.