Assembly is mentioned as important process saving time and cost where we produce the machine with many parts relationships. In this study, parts assembly relationship is analysed for assembly information of micro milling machine which have been developing for research. Liaison diagram, datum flow chain and assembly tree are applied to discuss assembly characteristics of micro milling machine model. We can find out the characteristics of micro machine assembly and discuss about facility of assembly. Some analysis in this paper about micro milling machine will give a useful tools for assembly. We knew that the predicted results from analysis in this study are alignment and clearance among the parts. The 3D model of micro machine which is studied in this paper is not a complete model. Main parts of a micro milling machine are used and presented.