The purpose of this study was to examine the differences of lower limbs muscle activities depending on three walking speeds of 2.5km/h, 5.0km/h and 7.5km/h during forward walking and backward walking making 14 students the subjects of this study. To achieve this aim, surface electrodes for factor analysis of EMG were adhered to rectus femoris, biceps femoris, tibialis anterior and gastrocnemius medial head of right lower limbs. The conclusions through this study are as follows. 1) The muscle activity of rectus femoris was higher in backward walking group than in forward walking group and it was the highest at 7.5km/h walking speed. 2) The muscle activity of biceps femoris was higher in forward walking group than in backward walking group. It was the lowest at 5.0km/h walking speed and the highest at 7.5km/h walking speed. 3) The muscle activity of tibialis anterior was higher in backward walking group than in forward walking group. It was the lowest at 5.0km/h walking speed and the highest at 7.5km/h walking speed. 4) The muscle activity of gastrocnemius medial head was higher in backward walking group than in forward walking group except P2. It was the lowest at 5.0km/h walking speed and the highest at 7.5km/h walking speed.