The effects of temperature heating-up rate and pressure building-up phase on the inactivation of Listeria innocua ATCC 33090 were evaluated in buffered peptone water. The number of L. innocua was reduced by 5.57 and 6.52 log CFU/mL during the nonisothermal treatment (the come-up time followed by isothermal process) and the isothermal treatment, respectively, at $60^{circ}C$. When compared to the isothermal treatment (0.76<D value<0.96), L. innocua exposed at the nonisothermal treatment (1.58<D value<2.31) became more resistant to heat. The come-up time reductions in numbers of L. innocua significantly increased with increasing the heating rates (p<0.05). The pronounced reduction was observed by more than 5 log CFU/mL at $33.2^{circ}C/min$ of temperature heating-rate. The effect of the combined high pressure and thermal processing on the inactivation of L. innocua increased with increasing pressure and temperature. At all temperature levels from 40 to $60^{circ}C$ under 700 MPa, L. innocua was not detected by enrichment culture (>7 log reduction).