This paper investigates on the use of constant-amplitude zero-autocorrelation (CAZAC) sequence for channel estimation in multiple-input multiple-output (MIMO) system over indoor wireless channel. Since the symbol-length of the conventional 4-phase CAZAC sequence is short, there is a limitation to use it for MIMO system in multipath environments. An algorithm which generates longer CAZAC sequences is proposed to overcome that problem. Flexible symbol-length of 4-phase CAZAC sequences can be made by the proposed algorithm. Therefore appropriate symbol-length of CAZAC sequences could be utilized as preambles in accordance with the number of transmit antennas and channel condition. The effect of the number of CAZAC sequences for channel estimation is presented in terms of mean square error (MSE).