Elastic-plastic structural analysis for regenerative cooling chamber of gas generator was performed. Uniaxial tension test was conducted for STS316L at room and high temperature conditions to get the material data necessary for the structural analysis of the chamber which was operated under thermal load and high internal pressure. Physical properties including thermal conductivity, specific heat and thermal expansion were also measured. The structural analysis for four different types of regenerative cooling chamber of gas generator revealed that increased cooling performance decreased the thermal load and strain of the cooling channel structure. The results propose that in order for the regenerative cooling gas generator chamber to have high structural stability with endurance to high mechanical and thermal loads, it is important for the chamber to be designed to have high cooling performance.