A simple reversed phase HPLC method was developed for extracting pharmacologically active compounds kurarinone and trifolirhizin from the roots of Sophora flavescens using a binary gradient of acetonitrile : $H_{2}O$ with UV detection at 254 nm. The kurarinone and trifolirhizin contents of the roots of S. flavescens collected from ten district markets in Korea and China were $77.24{sim}686.89$ ${mu}g/g$ and $46.89{sim}288.58$ ${mu}/g$, respectively.