The purpose of this research was to investigate the anti-inflammatory effects of Kamibokwontonggi-san(KBTS) which has been medicated the patient such as mastitis, mammary tuberculosis. KBTS in RAW264.7 cell inhibited IL-1 ${eta}$, IL-6, TNF-${alpha}$, COX-2 and NOS-II mRNA genes expression in a concentration-dependent manner. KBTS inhibited NO production significantly at 100, 50 ${mu}g/m{ell}$ and ROS production in a concentration-dependent manner. KBTS inhibited IL-1${eta}$, IL-6 and TNF-${alpha}$ production significantly in serum of acute anti-inflammation-induced mice and the survival rate at the 3rd day on LPS-induced lethal endotoxemia. These results suggest that Kamibokwonntonggi-san (KBTS) can be useful in treating a lot of women diseases caused by inflammation such as mastitis, mammary tuberculosis, pelvic inflammatory disease and pelvic tuberculosis.